Cryo-Electron Microscopy Senior Scientist in Blue Sky Protein Kinases

Company: St. Jude Children's Research Hospital
Location: Memphis
Posted on: May 17, 2025

Job Description:

We are looking for an experienced cryo - electron microscopy (CryoEM) senior scientist to participate in our exciting studies of the human protein kinome. As part of the largest BlueSky initiative, "Seeing the Invisible in Protein Kinases" at St. Jude Children's Research Hospital, we are expanding our structural studies of the human kinome to identify novel kinase-binding ligands. We are looking for experienced CryoEM scientist to aid in these efforts. The World's Brightest Minds Always Innovate - - At St. Jude Children's Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That's why we provide world-class facilities. State-of-the-art technologies. Extraordinary support. And a collaborative, bench-to-bedside environment where you can see, firsthand, how your science translates into survival for the children we serve. Quite simply, at St. Jude, we encourage you to dream big and stop at nothing when it comes to finding cures and saving children. - The -Cryo-EM Center -at St. Jude is at the forefront of scientific discovery, supporting its scientists and investigators in their research pursuits. The Center offers an entire Single Particle Analysis (SPA) solution and has rapidly progressed into the domain of Cryo-Electron Tomography (CryoET). Recently, the Center acquired cutting-edge instrumentation, including a Titan Krios G4 electron microscope (TEM) featuring a 'cold-FEG' electron source, a SelectrisX energy filter, and a Falcon 4i camera. Moreover, the Center is supported by St. Jude's high-performance computing facility with dedicated GPU and CPU nodes and a larger shared filesystem. The research activity will be supported by Blue Sky initiative. This role will involve working closely as part of a multidisciplinary team including biochemists, NMR spectroscopists, structural biologists, computational chemists, and other biophysical specialists. Ideal Candidate:

• Critically evaluate the feasibility of Protein Kinase mediated complexes for CryoEM studies.
• Experience in CryoEM workflow, structure determination of human protein cell signaling complexes. Strong command of CryoEM/ET data processing.
• Previous experience in working with a multidisciplinary team supporting and managing multiple projects simultaneously.
• At least few years of experience in the vitrification of biological samples using semi-automated devices such as Vitrobot III or IV, Leica GP2 plunger.
• At least three years of experience independently operating an Arctica, a Talas, or a Titan Krios electron microscope.
• Proficiency in SPA or tilt series data collection using programs such as EPU, TOMO5, and SerialEM.
• Hands-on experience with image processing and using one or two programs, such as RELION, cryoSPARC, EMAN2, and model analysis software, such as COOT and Chimera. Key Responsibilities
  • Extensive experience in applying CryoEM techniques to determine the structure of protein complexes.
  • Support the CryoEM projects of the team, by working closely with other team members.
  • Coordinate with the leaders of the project to design CryoEM studies with specific goals objectives and experimental plan.
  • Prepare grids for samples provided by researchers across the team.
  • Implement workflows that will help the researchers with their CryoEM studies.
  • Provide training to the team members on instrument operation and sample preparation techniques.
  • Independently troubleshoot, solve difficult problems, and propose solutions related to research project design and execution
  • Work effectively in a collaborative team environment where results are achieved through influence, cooperation, and the incorporation of multiple points of view
  • Perform additional duties as assigned, such as performing FIB-milling. Training can be provided.
  • Stay well-aware about the progress in the field of Human kinases and should present new findings in journal clubs and department seminars.
  • Independently motivated, detail oriented and good problem-solving ability
  • Excellent organizational skills, with an ability to embrace change and multi-task in an extremely fast-paced environment
  • Excellent communications skills Minimum Education and/or Training:
    • Bachelor's degree in relevant scientific area is required.
    • PhD preferred. Minimum Experience:
      • Ten (10) years relevant work experience is required with a Bachelor's degree.
      • Eight (8) years of relevant work experience is required with a Master's degree.
      • Five (5) years of relevant work experience is required with a PhD. Have More in Memphis Get the best of big-city amenities mixed with all the charm of a small-town feel when you live in Memphis. From our world-renowned music scene to our eclectic mix of food and people. Memphis is a place our employees are proud to call home. Our region enjoys a cost of living more than 20% lower than the national average and our state places no personal income tax on wages. Plus, Memphians spend five hours less per year in their daily commute compared to the national average. Learn how you can "Have More in Memphis." - Compensation In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $104,000 - $186,160 per year for the role of Cryo-Electron Microscopy Senior Scientist in Blue Sky Protein Kinases. Explore our exceptional benefits! St. Jude is an Equal Opportunity Employer No Search Firms St. Jude Children's Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.

Keywords: St. Jude Children's Research Hospital, Jonesboro , Cryo-Electron Microscopy Senior Scientist in Blue Sky Protein Kinases, Other , Memphis, Arkansas